1N0E
CRYSTAL STRUCTURE OF A CELL DIVISION AND CELL WALL BIOSYNTHESIS PROTEIN UPF0040 FROM MYCOPLASMA PNEUMONIAE: INDICATION OF A NOVEL FOLD WITH A POSSIBLE NEW CONSERVED SEQUENCE MOTIF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.627, 100.064, 100.851 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.106 * | 0.390 * |
| Number of reflections | 42426 * | 2068 * |
| <I/σ(I)> | 18.3 | 3.55 |
| Completeness [%] | 98.6 * | 97.5 |
| Redundancy | 7.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 28 (mg/ml) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | drop | Tris-HCl | 0.1 (%) | pH8.5 |
| 4 | 1 | drop | PEG3350 | 20 (%(w/v)) | |
| 5 | 1 | drop | glycerol | 20 (%) |
