1N06
Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 |
Unit cell lengths | 38.979, 45.613, 52.105 |
Unit cell angles | 91.11, 111.22, 97.04 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.27300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n05 |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 | 0.412 |
Number of reflections | 21249 | |
Completeness [%] | 95.1 | 92.4 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 20 * | PEG 8000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | reservoir | MES-NaOH | 0.1 (M) | pH6.5 |
5 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
6 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |