1MZZ

Crystal Structure of Mutant (M182T)of Nitrite Reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2000-08-02
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	92.495, 123.729, 130.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.170
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.157
Number of reflections	92678
<I/σ(I)>	6.9
Completeness [%]	91.4	50.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	(NH4)2SO4, NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K