1MZY
Crystal Structure of Nitrite Reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.018 |
Spacegroup name | H 3 |
Unit cell lengths | 71.392, 71.392, 146.246 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.540 - 1.460 |
Rwork | 0.185 |
R-free | 0.20990 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.540 | 1.540 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.204 | 0.410 |
Number of reflections | 48257 | |
<I/σ(I)> | 2.5 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.2 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 6000 or 4000, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |