1MZW
Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.369, 59.526, 47.040 |
Unit cell angles | 90.00, 110.65, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 * |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qoi |
RMSD bond length | 0.005 |
RMSD bond angle | 1.280 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.100 |
High resolution limit [Å] | 2.100 * | 2.100 * |
Rmerge | 0.063 * | 0.236 * |
Total number of observations | 55492 * | |
Number of reflections | 13762 * | |
Completeness [%] | 99.2 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 21 * | PEG 8000, magnesium acetate, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 2 (%(w/v)) | |
2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH6.5 |