1MZU

Crystal Structure of the Photoactive Yellow Protein Domain from the Sensor Histidine Kinase Ppr from Rhodospirillum centenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	293
Detector technology	CCD
Collection date	2001-09-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	34.560, 143.070, 36.450
Unit cell angles	90.00, 117.96, 90.00

Refinement procedure

Resolution	40.000 * - 2.000
R-factor	0.247
Rwork	0.245
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.540 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.080 *	0.379 *
Number of reflections	19047 *
<I/σ(I)>		3.7
Completeness [%]	93.2	78.9 *
Redundancy	4.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	36% PEG 6K, 10 mM Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG6000	36 (%)
3	1	reservoir	MES	10 (mM)	pH7.0