1MZP
Structure of the L1 protuberance in the ribosome
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9762,0.9330 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 156.010, 156.010, 90.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.650 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.26600 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 20.600 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.057 | 0.376 |
Total number of observations | 277313 * | |
Number of reflections | 35675 * | |
Completeness [%] | 99.1 | 97.2 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 * | 4 * | PEG400, Tris HCl, KCl, MgCl2, ph 8.5, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | Tris HCl | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 2 | PEG 400 | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG400 | 10 (%(v/v)) | |
10 | 1 | reservoir | 100 (mM) | ||
11 | 1 | reservoir | 10 (mM) | ||
2 | 1 | drop | 50 (mM) | pH8.6 | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | 10 (mM) | ||
5 | 1 | drop | glycerol | 4 (%(v/v)) | |
6 | 1 | drop | RNA | 3 (mg/ml) | |
7 | 1 | drop | SacL1 | 4.8 (mg/ml) | |
8 | 1 | reservoir | PEG400 | 27 (%(v/v)) | |
9 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.5 |