1MZH
QR15, an Aldolase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798, 0.9799, 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.139, 123.458, 151.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30534 | |
| Completeness [%] | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG4000, ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
