1MZB
Ferric uptake regulator
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.2837, 1.2828, 1.1310 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 63.300, 63.300, 180.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.800 |
Rwork | 0.226 |
R-free | 0.25400 * |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.490 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.049 * | 0.425 * |
Total number of observations | 223710 * | |
Number of reflections | 17481 * | |
Completeness [%] | 99.8 * | 99.9 |
Redundancy | 12.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | ethyleneglycol, ZnSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ethylene glycol | 40 (%) | |
2 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |
3 | 1 | reservoir | 10 (mM) | ||
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | drop | Tris | 10 (mM) | pH7.5 |
6 | 1 | drop | 100 (mM) | ||
7 | 1 | drop | glycerol | 10 (%) | |
8 | 1 | drop | ascorbic acid | 1 (mM) |