1MZ8
CRYSTAL STRUCTURES OF THE NUCLEASE DOMAIN OF COLE7/IM7 IN COMPLEX WITH A PHOSPHATE ION AND A ZINC ION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.29004 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.735, 62.410, 74.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cei |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.660 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 * | 0.278 * |
| Total number of observations | 169490 * | |
| Number of reflections | 36897 | |
| <I/σ(I)> | 21.7 | 5.5 |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | PEG4000, sodium phosphate, ammonium acetate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | phosphate | 50 (mM) | pH6.3 |
| 3 | 1 | drop | ammonium acetate | 0.35 (M) | |
| 4 | 1 | drop | PEG4000 | 11 (%) | |
| 5 | 1 | reservoir | PEG4000 | 22.5 (%) |
