1MZ4

Crystal Structure of Cytochrome c550 from Thermosynechococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	P 32 2 1
Unit cell lengths	69.641, 69.641, 92.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.580 - 1.800
R-factor	0.182
Rwork	0.182
R-free	0.20200
Structure solution method	two wavelength anomalous dispersion
RMSD bond length	0.006
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	25953
<I/σ(I)>	25.2	4.6
Completeness [%]	98.2	96.7
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2 M dihydrogen phosphate, MES, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K