1MYL

SUBSTITUTING HYDROPHOBIC RESIDUES FOR A BURIED SALT BRIDGE ENHANCES PROTEIN STABILITY BUT DOES NOT REDUCE CONFORMATIONAL SPECIFICITY

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	29.600, 117.100, 49.700
Unit cell angles	90.00, 98.60, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.209
Rwork	0.209
R-free	0.29300
RMSD bond length	0.011
RMSD bond angle	1.550
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.062 *
Total number of observations	20111 *
Number of reflections	11168
Completeness [%]	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	1	bis-Tris-propane-Cl	30 (mM)
3	1	1		1 (mM)
4	1	2	PEG3400	19-22 (%)
5	1	2	Tris-HCl	100 (mM)
6	1	2	Na-acetate	200 (mM)