1MXX
crystal titration experiments (AMPA co-crystals soaked in 100 uM BrW)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91843 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.538, 163.761, 48.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.890 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | BrW complex in the Zn form |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.203 |
| Number of reflections | 61303 | |
| Completeness [%] | 98.3 | 94.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG8000, zinc acetate, sodium cacodylate, pH 6.5 and AMPA, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.25-0.3 (M) | |
| 7 | 1 | reservoir | zinc acetate | 0.05 (M) | |
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
