1MXC
S-ADENOSYLMETHIONINE SYNTHETASE WITH 8-BR-ADP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 128.900, 128.900, 139.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAT ITSELF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 3.050 |
| Data reduction software | ELMS |
| Data scaling software | KUAVST |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.069 | 0.220 |
| Total number of observations | 56509 * | |
| Number of reflections | 13244 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 95.8 | 90.1 |
| Redundancy | 4 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | potassium phosphate | 100 (mM) | |
| 2 | 1 | 1 | 10 (mM) | ||
| 3 | 1 | 1 | ammonium sulfate | 33 (%(v/v)sat) | |
| 4 | 1 | 1 | protein | 10 (mg/ml) |
