1MXB
S-ADENOSYLMETHIONINE SYNTHETASE WITH ADP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 128.900, 128.900, 139.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAT ITSELF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.500 * |
| Data scaling software | KUAVST |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.053 | 0.220 |
| Total number of observations | 51217 * | |
| Number of reflections | 16033 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 94.4 | 87.1 |
| Redundancy | 5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 26 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | potassium phosphate | 100 (mM) | |
| 2 | 1 | 1 | ATP | 10 (mM) | |
| 3 | 1 | 1 | 10 (mM) | ||
| 4 | 1 | 1 | ammonium sulfate | 33 (%(v/v)sat) | |
| 5 | 1 | 1 | protain | 10 (mg/ml) |
