1MX0
Structure of topoisomerase subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 146.655, 219.192, 106.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.21854 |
| Rwork | 0.214 |
| R-free | 0.26300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.423 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.111 * | 0.473 * |
| Total number of observations | 2006747 * | |
| Number of reflections | 149698 * | |
| <I/σ(I)> | 10 | 2.24 |
| Completeness [%] | 96.1 * | 88.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 * | 20 * | PEG 3000, Sodium Citrate, Magnesium Chloride, AMP-PNP, pH 5.5, Microbatch, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | pH7.0 |
| 2 | 1 | 1 | 100 (mM) | ||
| 3 | 1 | 1 | protein | 12 (mg/ml) | |
| 4 | 1 | 1 | PEG3000 | 20 (%) | |
| 5 | 1 | 2 | Tris-HCl | 100 (mM) | pH7.0 |
| 6 | 1 | 2 | 200 (mM) |
