1MWM
ParM from plasmid R1 ADP form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9600 |
Spacegroup name | P 41 |
Unit cell lengths | 64.710, 64.710, 199.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 2.000 |
R-factor | 0.2224 |
Rwork | 0.221 |
R-free | 0.25200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Semet ParM |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.381 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 21.900 |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.064 * |
Redundancy | 4.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 4 * | 100mM Na-cacodyltae, 400mM NaCl, 400mM Mg-acetate, 11% PEG8000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.2 |
2 | 1 | reservoir | 400 (mM) | ||
3 | 1 | reservoir | magnesium acetate | 400 (mM) | |
4 | 1 | reservoir | PEG8000 | 11 (%) | |
5 | 1 | drop | protein | 10 (mg/ml) | |
6 | 1 | drop | ADP | 1 (mM) | |
7 | 1 | drop | 1 (mM) |