1MWL
Crystal structure of geneticin bound to the eubacterial 16S rRNA A site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.200, 33.180, 51.740 |
Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.400 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1LC4 (RNA ONLY) |
RMSD bond length | 0.007 |
RMSD bond angle | 10.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.062 * | 0.211 * |
Total number of observations | 24145 * | |
Number of reflections | 5466 | 566 * |
<I/σ(I)> | 39.3 | 6.7 |
Completeness [%] | 88.8 | 92.8 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 37 * | MPD, MAGNESIUM SULPHATE, POTASSIUM CHLORIDE, SODIUM CHLORIDE, SODIUM CACODYLATE, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 37 * | MPD, MAGNESIUM SULPHATE, POTASSIUM CHLORIDE, SODIUM CHLORIDE, SODIUM CACODYLATE, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MPD | 1.5-3.5 (%) | |
2 | 1 | drop | glycerol | 1.0-2.0 (%(v/v)) | |
3 | 1 | drop | geneticin | 2 (mM) | |
4 | 1 | drop | 150 (mM) | ||
5 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.4 |
6 | 1 | reservoir | MPD | 40 (%) |