1MWK

ParM from plasmid R1 APO form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 21 21 2
Unit cell lengths	117.680, 72.460, 87.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.300
R-factor	0.2196
Rwork	0.217
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.193 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.500
High resolution limit [Å]	2.300
Rmerge	0.064 *
Number of reflections	31145
Completeness [%]	98.9
Redundancy	3.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	19 *	1.5M Na3-citrate, 90mM Guanidinium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.4-1.6 (M)	pH6.5
2	1	reservoir	guanidine-HCl	80-100 (mM)
3	1	drop	protein	10 (mg/ml)