1MWJ
Crystal Structure of a MUG-DNA pseudo substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 101.996, 101.996, 45.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.850 |
| R-factor | 0.23 |
| Rwork | 0.190 |
| R-free | 0.25000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native MuG |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.087 | 0.278 * |
| Number of reflections | 5479 * | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 94.5 | 96.7 * |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 4.6 | 289 | Barrett, T.E., (1998) Cell., 92, 117. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | Sodium Acetate | ||
| 3 | 1 | 2 | Sodium Acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 2 | 1 | Tris-HCl | 20 (mM) | pH8.0 | |
| 3 | 1 | PEG4000 | 8 (%) | ||
| 4 | 1 | 2 | sodium acetate | 0.1 (M) | pH4.6 |
