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1MWH

REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG

Experimental procedure
実験手法SINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]100
Detector technologyCCD
Collection date2000-12-20
DetectorADSC QUANTUM 4
Wavelength(s)1.000
Spacegroup nameP 21 21 21
格子定数 [Å]70.891, 84.944, 249.246
格子定数 [度]90.00, 90.00, 90.00
精密化法
残基30.000

*

- 2.500
R因子0.243
Rwork0.209
R-free0.25200

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)reovirus polymerase 1MUK
結合長の平均二乗偏差(RMSD) [Å]0.009
結合角の平均二乗偏差(RMSD) [度]1.719

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.0)
Quality characteristics
 OverallOuter shell
分解能 [Å] (低)30.000

*

2.590
分解能 [Å] (高)2.500

*

2.500
Rmerge_l_obs0.088

*

0.404

*

独立反射数49606
<I/σ(I)>152
完全性 [%]93.3

*

80.8

*

冗長性5-6

*

2
結晶化条件
結晶ID方法pH温度溶液条件
1VAPOR DIFFUSION, HANGING DROP7.822

*

PEG4000, sodium chloride, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
文献の結晶化試薬
ID結晶ID溶液試薬名濃度 (単位) (単位)詳細
11dropprotein8.7 (mg/ml)
21dropsodium HEPES50 (mM)pH7.8
31dropglycerol10 (%)
41drop150 (mM)
51dropdithiothreitol10 (mM)
61drop0.02 (%)
71reservoirPEG80007.5 (%)
81reservoirglycerol10 (%)
91reservoirsodium HEPES0.1 (M)pH7.8

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件を2024-09-18に公開中

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