1MW0
Amylosucrase mutant E328Q co-crystallized with maltoheptaose then soaked with maltoheptaose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2001-02-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.008 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.067, 116.097, 60.745 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.840 - 2.000 * |
Rwork | 0.185 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 22.500 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 * | 0.301 * |
Total number of observations | 403150 * | |
Number of reflections | 45913 | |
Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Skov, L.K., (2000) Acta Crystallogr., D56, 203. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4-6 (mg/ml) | |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | alpha-dithiothreitol | 1 (mM) | |
6 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
7 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |