1MVQ
Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.38 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.449, 77.956, 105.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.940 - 1.770 |
| R-factor | 0.14173 |
| Rwork | 0.140 |
| R-free | 0.17000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cna |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.599 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.09) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.940 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.068 * | 0.345 * |
| Number of reflections | 25553 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 3.8 * | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 291 * | Tavares, G.A., (1996) Acta Crystallogr., D52, 1046. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.01 (M) | pH4.5 |
| 3 | 1 | reservoir | 0.15 (M) | ||
| 4 | 1 | reservoir | PEG6000 | 9 (%(w/v)) | |
| 5 | 1 | reservoir | 0.01 (M) | ||
| 6 | 1 | reservoir | methyl-alpha-D-mannopyranoside | 0.15 (M) |
