1MVO
Crystal structure of the PhoP receiver domain from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.704, 45.704, 134.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.100 * - 1.600 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Metal-free PhoPN structure solved by MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 * | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | 0.187 |
| Total number of observations | 224913 * | |
| Number of reflections | 18249 * | |
| <I/σ(I)> | 5.2 | 3.5 |
| Completeness [%] | 92.0 * | 92 |
| Redundancy | 6.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 * | PEG 10000, sodium citrate, PEG MME 550, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG10000 | 23-25 (%) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.8 |
| 3 | 1 | reservoir | PEG550 MME | 3 (%) | |
| 4 | 1 | drop | Tris | 20 (mM) | pH7.8 |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | dithiothreitol | 1 (mM) |
