1MVH

structure of the SET domain histone lysine methyltransferase Clr4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-20
Detector	BRANDEIS - B1.2
Wavelength(s)	1.1
Spacegroup name	P 61 2 2
Unit cell lengths	75.070, 75.070, 190.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.225
R-free	0.26800 *
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.340 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.049 *
Total number of observations	386726 *
Number of reflections	14740 *
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	289	ammonium sulfate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	5 (mM)	pH8.0
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	ammonium sulfate	0.7-1.5 (M)