1MVF
MazE addiction antidote
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.440, 47.826, 128.376 |
| Unit cell angles | 90.00, 90.59, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.2203 |
| Rwork | 0.218 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.228 * |
| Total number of observations | 96388 * | |
| Number of reflections | 32375 * | 2633 * |
| <I/σ(I)> | 11.82 | |
| Completeness [%] | 74.1 | 61 * |
| Redundancy | 2.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 293 | PEG 8000, magnesium acetate, cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | Vapor diffusion * | 6.5 | 293 | PEG 8000, magnesium acetate, cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | magnesium acetate | 0.2 (M) |
