1MV5

Crystal structure of LmrA ATP-binding domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-08
Detector	MARRESEARCH
Wavelength(s)	0.94
Spacegroup name	P 63
Unit cell lengths	141.866, 141.866, 120.965
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.100
R-factor	0.244
Rwork	0.251
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	2.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	24919
Completeness [%]	1.0	0.99
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG8000, cacodylate, CaCl2, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG8000, cacodylate, CaCl2, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG8000, cacodylate, CaCl2, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K