Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1999-12-17 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.70090,0.97910,0.97630, 0.72130 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 113.700, 113.700, 228.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.25700 * |
Structure solution method | MAD |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.795 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.300 | |
Rmerge | 0.053 | 0.323 * |
Number of reflections | 39476 | |
<I/σ(I)> | 25.56 | |
Completeness [%] | 99.6 | 98.2 * |
Redundancy | 14.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.7 * | 290 | PEG400, Succinate, pH 6.00, VAPOR DIFFUSION, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG400 | ||
2 | 1 | 1 | Succinate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 0.3 (M) | ||
2 | 1 | drop | HEPES | 20 (mM) | pH7.7 |
3 | 1 | drop | EDTA | 2 (mM) | |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | PEG400 | 30 (%(w/v)) | |
6 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
7 | 1 | reservoir | ammonium sulfate | 60 (mM) |