Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1999-12-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.70090,0.97910,0.97630, 0.72130 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 113.700, 113.700, 228.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.795 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.053 | 0.323 * |
| Number of reflections | 39476 | |
| <I/σ(I)> | 25.56 | |
| Completeness [%] | 99.6 | 98.2 * |
| Redundancy | 14.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.7 * | 290 | PEG400, Succinate, pH 6.00, VAPOR DIFFUSION, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | Succinate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.3 (M) | ||
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.7 |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG400 | 30 (%(w/v)) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 7 | 1 | reservoir | ammonium sulfate | 60 (mM) |
