1MUF

Structure of histone H3 K4-specific methyltransferase SET7/9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-18
Detector	SBC-2
Wavelength(s)	0.95372,0.9791,0.97921
Spacegroup name	P 64 2 2
Unit cell lengths	110.973, 110.973, 96.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.220 - 2.260
R-factor	0.229
Rwork	0.229
R-free	0.23000 *
Structure solution method	MAD
RMSD bond length	0.032 *
RMSD bond angle	2.200 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.340
High resolution limit [Å]	2.260	2.260
Rmerge	0.096 *
Number of reflections	30900 *	3074 *
Completeness [%]	100.0 *	100 *
Redundancy	10.7 *	8.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	10 *	PEG 3000, CaCl2, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	imidazole	20 (mM)	pH7.0
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	Tris-HCl	0.1 (M)	pH7.0
6	1	reservoir		0.2 (M)
7	1	reservoir	PEG3000	19 (%(w/v))