1MUF
Structure of histone H3 K4-specific methyltransferase SET7/9
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-18 |
Detector | SBC-2 |
Wavelength(s) | 0.95372,0.9791,0.97921 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 110.973, 110.973, 96.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.220 - 2.260 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.23000 * |
Structure solution method | MAD |
RMSD bond length | 0.032 * |
RMSD bond angle | 2.200 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.096 * | |
Number of reflections | 30900 * | 3074 * |
Completeness [%] | 100.0 * | 100 * |
Redundancy | 10.7 * | 8.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | PEG 3000, CaCl2, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
6 | 1 | reservoir | 0.2 (M) | ||
7 | 1 | reservoir | PEG3000 | 19 (%(w/v)) |