1MU4
CRYSTAL STRUCTURE AT 1.8 ANGSTROMS OF THE BACILLUS SUBTILIS CATABOLITE REPRESSION HISTIDINE CONTAINING PROTEIN (CRH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 68.850, 68.850, 119.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mo1 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.318 |
| Number of reflections | 27478 * | |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 99.6 | 99.6 * |
| Redundancy | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 18 * | ammonium sulfate, peg 1000,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 3 | 1 | reservoir | PEG1000 | 2 (%(v/v)) | |
| 4 | 1 | reservoir | 20 (mM) | pH6.5 | |
| 5 | 1 | drop | 20 (mM) |
