1MU0

Crystal Structure of the Tricorn Interacting Factor F1 Complex with PCK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-01-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.579, 61.721, 80.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.366
Rwork	0.314
R-free	0.36600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mt3
RMSD bond length	0.004
RMSD bond angle	0.948 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400 *
Number of reflections	11344 *
<I/σ(I)>	3.2
Completeness [%]	85.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	293	12% PEG 6000, 100 mM Bis-Tris-HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	reservoir	Bis-Tris-HCl	100 (mM)	pH6.0
4	1	reservoir	PEG6000	7-12 (%)
5	1	reservoir	HEPES	100 (mM)	pH7.5
6	1	reservoir	PEG8000	20 (%)