1MST
CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 0.87,0.93,0.95 |
Spacegroup name | H 3 2 |
Unit cell lengths | 288.000, 288.000, 653.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
R-factor | 0.218 |
Rwork | 0.218 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.590 * | |
High resolution limit [Å] | 2.600 * | 2.600 * |
Rmerge | 0.115 | 0.313 * |
Number of reflections | 247689 | 10886 * |
Completeness [%] | 79.0 * | 47 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | 37 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MS2 | 1.0 (%(w/v)) | |
2 | 1 | drop | sodium phosphate | 0.2 (M) | |
3 | 1 | drop | PEG6000 | 1.5 (%(w/v)) | |
4 | 1 | drop | 0.02 (%(w/v)) | ||
5 | 1 | reservoir | sodium phospahte | 0.4 (M) |