1MSO
T6 Human Insulin at 1.0 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1997-11-01 |
| Detector | BRUKER SMART 2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.286, 81.286, 33.714 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.400 - 1.000 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.011 |
| RMSD bond angle | 20.800 * |
| Data reduction software | SORTAV |
| Data scaling software | SORTAV |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.049 | 0.369 |
| Total number of observations | 72631 * | |
| Number of reflections | 41170 | |
| <I/σ(I)> | 11 | 2.3 |
| Completeness [%] | 91.7 | 75 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.3 | 298 | 0.001 M HCl, 0.007 M Zinc Acetate, 0.05 M Sodium Citrate, 17% acetone, pH 6.3, SLOW COOLING at 298K, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 5 (mg/ml) | |
| 2 | 1 | 1 | 0.01 (M) | ||
| 3 | 1 | 1 | zinc acetate | 0.007 (M) | |
| 4 | 1 | 1 | sodium citrate | 0.05 (M) | |
| 5 | 1 | 1 | acetone | 17 (%) | pH6.3 |
