1MSD
COMPARISON OF THE CRYSTAL STRUCTURES OF GENETICALLY ENGINEERED HUMAN MANGANESE SUPEROXIDE DISMUTASE AND MANGANESE SUPEROXIDE DISMUTASE FROM THERMUS THERMOPHILUS. DIFFERENCES IN DIMER-DIMER INTERACTIONS.
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.800, 74.100, 68.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 3.200 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 3.4 (mg/ml) | |
| 2 | 1 | drop | ammonium phosphate | ||
| 3 | 1 | drop | MPD | 10 (%) | |
| 4 | 1 | reservoir | MPD | 32 (%) |
