1MSC

CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER

Experimental procedure

Collection date	1993-12-07
Spacegroup name	P 21 21 2
格子定数 [Å]	76.200, 55.700, 28.400
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	8.000 - 2.000
R因子	0.2
結合長の平均二乗偏差(RMSD) [Å]	0.021
結合角の平均二乗偏差(RMSD) [度]	0.066
Data reduction software	XENGEN ((HOWARD)
Refinement software	PROLSQ

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	55.000
分解能 [Å] (高)	2.030	2.030 *
Rmerge_l_obs	0.031	0.107 *
Total number of observations	23935 *
独立反射数	7447	1033 *
完全性 [%]	90.0	65 *
冗長性	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	6.2 *		COMPND MOLECULE: MS2 UNASSEMBLED COAT PROTEIN DIMER. THIS BACTERIOPHAGE COAT PROTEIN WAS CRYSTALLIZED AS AN UNASSEMBLED DIMER; IT DID NOT FORM VIRAL CAPSIDS.

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	PEG8000	10 (%)
2	1	drop	protein	3 (mg/ml)
3	1	reservoir	citrate-phosphate	100 (mM)