1MRI
STUDIES ON CRYSTAL STRUCTURES ACTIVE CENTER GEOMETRY AND DEPURINE MECHANISM OF TWO RIBOSOME-INACTIVATING PROTEINS
Experimental procedure
Spacegroup name | H 3 |
Unit cell lengths | 130.970, 130.970, 40.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.200 |
R-factor | 0.192 |
Rwork | 0.192 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.450 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Rmerge | 0.062 * |
Number of reflections | 11731 * |
Completeness [%] | 83.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (%(w/v)) | |
2 | 1 | reservoir | PEG6000 | 14 (%(w/v)) | |
3 | 1 | reservoir | sodium citrate | 0.3 (M) |