1MRH
STUDIES ON CRYSTAL STRUCTURES ACTIVE CENTER GEOMETRY AND DEPURINE MECHANISM OF TWO RIBOSOME-INACTIVATING PROTEINS
Experimental procedure
Spacegroup name | H 3 |
Unit cell lengths | 131.600, 131.600, 39.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.000 |
R-factor | 0.191 |
Rwork | 0.191 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.410 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.072 * |
Number of reflections | 13935 * |
Completeness [%] | 72.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (%(w/v)) | |
2 | 1 | reservoir | 26 (%(w/v)) | ||
3 | 1 | reservoir | sodium citrate | 0.75 (M) | |
4 | 1 | reservoir | ligands | 2 (mM) |