1MRG

STUDIES ON CRYSTAL STRUCTURES ACTIVE CENTER GEOMETRY AND DEPURINE MECHANISM OF TWO RIBOSOME-INACTIVATING PROTEINS

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	131.600, 131.600, 39.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 1.800
R-factor	0.195
Rwork	0.195
RMSD bond length	0.012
RMSD bond angle	1.620
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.083 *
Number of reflections	14716 *
Completeness [%]	61.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%(w/v))
2	1	reservoir		26 (%(w/v))
3	1	reservoir	sodium citrate	0.75 (M)
4	1	reservoir	ligands	2 (mM)