1MRA
MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-07-25 |
| Detector | SIEMENS |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 125.000, 125.000, 107.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.19 * |
| RMSD bond length | 0.013 |
| RMSD bond angle | 25.000 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.171 |
| Number of reflections | 24713 |
| <I/σ(I)> | 1 |
| Completeness [%] | 74.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Mitra, B., (1995) Biochemistry, 34, 2777. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 50 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | (S)-atrolactate | 50 (mM) | |
| 4 | 1 | drop | D270N | 13 (mg/ml) | |
| 5 | 1 | drop | atrolactate | 5 (mM) | |
| 6 | 1 | reservoir | 30 (%) | ||
| 7 | 1 | reservoir | acetone | 3 (%(v/v)) |
