1MR9

Crystal structure of Streptogramin A Acetyltransferase with acetyl-CoA bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2001-03-14
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	F 2 2 2
Unit cell lengths	181.516, 184.530, 186.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.24557
Rwork	0.243
R-free	0.29500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mr7
RMSD bond length	0.011
RMSD bond angle	1.630 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.070
High resolution limit [Å]	3.000	3.000
Rmerge	0.080 *	0.353 *
Total number of observations	173203 *
Number of reflections	31022
<I/σ(I)>	11.4	2.25
Completeness [%]	96.9 *	98 *
Redundancy	3.18 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	2.9M Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	protein	0.15-0.25 (mM)
2	1	reservoir	Tris-HCl	1 (mM)	pH7.5