1MQS

Crystal structure of Sly1p in complex with an N-terminal peptide of Sed5p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-15
Detector	MARRESEARCH
Wavelength(s)	0.9875,0.9793,0.9789,0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	161.056, 161.056, 88.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000
Rwork	0.257
R-free	0.29040
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	22.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.044	0.552
Total number of observations	200527 *
Number of reflections	26182
<I/σ(I)>	13.3
Completeness [%]	100.0	100
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	3.5M sodium formate, 20mM Tris HCl pH 7.2, 50mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	3.5M sodium formate, 20mM Tris HCl pH 7.2, 50mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	sodium formate	3.4-3.6 (M)
3	1	drop	Tris-HCl	20 (mM)	pH7.2