1MQP

THE CRYSTAL STRUCTURE OF ALPHA-D-GLUCURONIDASE FROM BACILLUS STEAROTHERMOPHILUS T-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X13
Temperature [K]	203.4
Detector technology	CCD
Collection date	2000-09-07
Detector	MARRESEARCH
Wavelength(s)	0.913
Spacegroup name	P 41 21 2
Unit cell lengths	73.722, 73.722, 329.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.850 - 1.900
Rwork	0.192
R-free	0.22200
Structure solution method	AB INITIO PHASING
Starting model (for MR)	PDB CODE 1K9D
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.085	0.151
Number of reflections	67273
<I/σ(I)>	13.8
Completeness [%]	91.8	92.1
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	12% isopropanol, 14% PEG 4K, 0.1M Na-citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K