1MQN
BHA/LSTc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 147.322, 148.432, 250.248 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.200 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.155 * | 0.397 * |
Number of reflections | 44881 | |
Completeness [%] | 99.7 * | 99.9 * |
Redundancy | 7.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11.0 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
3 | 1 | reservoir | sodium citrate | 1.4 (M) | |
4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |