1MQ2
Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine and dAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.200, 81.000, 55.600 |
| Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.26 |
| Rwork | 0.257 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mq3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.119 | 0.255 |
| Number of reflections | 7613 | 507 * |
| <I/σ(I)> | 9.7 | 3.3 |
| Completeness [%] | 81.4 | 71.7 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 18 * | 20% PEG 3350, 350 mM sodium acetate, 50 mM imidazole at 18 degrees (C), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | imidazole |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 14-20 (%) | |
| 2 | 1 | reservoir | 350 (mM) | ||
| 3 | 1 | reservoir | 50 (mM) | pH7.5 | |
| 4 | 1 | drop | Bis-Tris | 20 (mM) | pH7.0 |
| 5 | 1 | drop | protein | 15 (mg/ml) |
