1MPY

STRUCTURE OF CATECHOL 2,3-DIOXYGENASE (METAPYROCATECHASE) FROM PSEUDOMONAS PUTIDA MT-2

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	FILM
Spacegroup name	P 42 21 2
Unit cell lengths	264.000, 264.000, 59.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.2
Rwork	0.200
R-free	0.28000
Structure solution method	MIR
RMSD bond length	0.022
RMSD bond angle	25.970 *
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.800
Rmerge	0.076
Number of reflections	46225
Completeness [%]	86.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	4 *	Kita, A., (1997) J.Biochem.(Tokyo), 122, 201. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium citrate	15 (%(w/v))
2	1	drop	protein	20 (mg/ml)
3	1	reservoir	precipitant	26-30 (%(w/v))