1MPT

CRYSTAL STRUCTURE OF A NEW ALKALINE SERINE PROTEASE (M-PROTEASE) FROM BACILLUS SP. KSM-K16

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	75.820, 57.790, 54.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.400
R-factor	0.189
Rwork	0.189
RMSD bond length	0.016
RMSD bond angle	0.038 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.119 *
Total number of observations	24185 *
Number of reflections	7897 *
Completeness [%]	80.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	291 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	acetate	50 (mM)
3	1	reservoir	acetate	50 (mM)
4	1	reservoir	ammonium sulphate	1.4 (M)