1MPS
PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M 197 REPLACED WITH ARG AND TYR M 177 REPLACED WITH PHE (CHAIN M, Y177F, F197R)
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-05 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 141.900, 141.900, 187.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 11.000 - 2.550 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.008 |
RMSD bond angle | 20.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.129 | 0.360 |
Number of reflections | 54163 | |
<I/σ(I)> | 8.8 | 2.7 |
Completeness [%] | 77.0 | 83 |
Redundancy | 3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 16 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium phosphate | 0.75 (M) | |
2 | 1 | drop | 1,4-dioxane | 2 (%(v/v)) | |
3 | 1 | drop | heptane-1,2,3-triol | 3.5 (%(w/v)) | |
4 | 1 | reservoir | potassium phosphate | 1.6 (M) |