1MPD
MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG), COMPLEXED WITH MALTOSE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-30 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.680, 58.960, 126.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.500 - 2.300 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.056 |
| Number of reflections | 18578 |
| Completeness [%] | 94.9 * |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 22 (%) | |
| 3 | 1 | drop | maltose | 100 (mM) | |
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | drop | sodium acetate | 200 (mM) | |
| 6 | 1 | drop | sodium azide | 5 (mM) |
