1MPC
MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG)
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-06 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.530, 58.350, 39.020 |
| Unit cell angles | 98.32, 102.85, 101.51 |
Refinement procedure
| Resolution | 7.500 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.045 |
| Number of reflections | 21467 |
| Completeness [%] | 88.8 * |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 25 (%) | |
| 3 | 1 | drop | sodium citrate | 10 (mM) | |
| 4 | 1 | drop | sodium azide | 5 (mM) |
