1MPA

BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-03
Detector	MAC Science DIP-2020
Spacegroup name	P 1 21 1
Unit cell lengths	55.680, 69.100, 72.950
Unit cell angles	90.00, 112.01, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.194
Rwork	0.194
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2HFL
RMSD bond length	0.009
RMSD bond angle	26.440 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.63)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.054	0.357
Total number of observations	94382 *
Number of reflections	15404
<I/σ(I)>	16.8	2.3
Completeness [%]	97.0	96.5
Redundancy	6.1	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		THE MN12H2 FAB PEPTIDE COMPLEX WAS CRYSTALLIZED FROM 15% W/V PEG 20000, 100 MM SODIUM ACETATE, PH 4.6, 20 MM CDCL.